In this study, COS of four different molecular weight ranges (<1 kDa, 1-3 kDa, 3-5 kDa and 5-10 kDa) were investigated for their abilities to modulates inflammatory mediators in lipopolysaccharides (LPS)-stimulated BV2 microglia. At the concentration of 500 �g/ml, COS attenuate the productions of nitric oxide (NO) and prostaglandin E2 (PGE2) by inhibiting inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. Furthermore, the release and expression levels of inflammatory cytokines; including tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6) and interleukin-1� (IL-1�) were also attenuated by COS. Notably, the inhibitory activity of COS depends significantly on its molecular weight, with lower molecular weight showed higher activity. Suppressive effects on the phosphorylation of JNK and p38 mitogen-activated protein kinase (MAPK) by COS were confirmed. These results indicate that COS could be used as an inhibitor in regulating microglial inflammatory responses. Moreover, COS may assist therapeutic treatment of neurodegenerative diseases.

Chitooligosaccharides inhibits lipopolysaccharides-induced iNOS and COX-2 expression in BV2 murine microglia cells

243rd National Meeting (2012)

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