Chemoenzymatic synthesis has long been of interest as a means to perform synthetic transformations on complex substrates.  Many enzymes use cofactors, which sometimes can be recycled conveniently by other enzymes.  In the case of (S)-adenosyl-L-methionine (SAM) an effective regeneration strategy has yet to be developed.  Following ATP, SAM is the second most used enzyme cofactor and it is responsible for a large number of biological reactions including methylation.  Utilizing an enzyme SAM can be produced in situ for in vitro methylation reactions.  This new methodology is effective for the concurrent production of SAM and methylation of either lambda phage DNA or the lipoglycopeptide antibiotic teicoplanin.

In situ synthesis of S-adenosyl-L-methionine and applications

243rd National Meeting (2012)

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