Aptamers are small single-strand DNA or RNA oligonucleotides with a very high selectivity and affinity for their targets, which can be screened from synthetic random oligonucleotides library by SELEX (systematic evolution of ligands by exponential enrichment) in vitro. Selection of aptamers against HBsAg would contribute to the detection and diagnosis of hepatitis B virus infection. In this work, we adopted negative SELEX method using microplate as matrix and prepared ssDNA library by separation method of Streptavidin-biotin magnetic beads for the next selection round. Binding affinity of aptamers with target HBsAg was determinated by ELOSA (Enzyme league oligonucleotide adsorption test) at 450 nm with microplate spectrophotometer. At last, in order to obtain individual aptamer sequence, the final selected product were cloned and sequenced. In conclusion, DNA aptamers that recognize HBsAg specifically was successed to obtain via 13 rounds selection.

Selection of DNA aptamers against HBsAg by SELEX

243rd National Meeting (2012)

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