Cytochrome P450 is an enzyme that catalyzes the oxidation of organic molecules. Recently, there has been a growing interest in the use of P450 as a biocatalyst for the production of pharmaceuticals because of its oxidative abilities. Cytochrome P450BM3 from Bacillus megaterium is relatively stable and has one of the highest catalytic activities in the P450 family.  Although the wild-type is only capable of oxidizing long-chain fatty acids, mutant types have been shown to oxidize a wider range of organic molecules. In a previous investigation conducted at Radboud University, the mutant P450 heme domain was uniformly labeled with 15N and analyzed with NMR spectroscopy. The results from this work suggested that selective labeling of amino acids would simplify the NMR spectra, in order to obtain more structural information. This study was performed to selectively label P450BM3MO2HD  with  15N-Ala, -Asn, and -Phe for NMR analysis.

Expression of <sup>15</sup>N selectively labeled P450BM3MO2HD for NMR spectroscopy

243rd National Meeting (2012)

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Expression of 15N selectively labeled P450BM3MO2HD for NMR spectroscopy

Expression of ¹⁵N selectively labeled P450BM3MO2HD for NMR spectroscopy