Engineering surfaces for adhesion and confinement of bacteria is interesting towards development of respective biosensors, and bio machine interfacing. Investigation was focused towards modification of surfaces towards confinement and entrapment of the pathogenic strain methicillin resistant Staphylococcus aureus (MRSA) or similar pathogenic strains and to study surface engineering. Clean, flat Au(111) on mica surfaces were used for self assembly for Self Assembled Monolayers (SAM). 1-hexanethiol, 1-octanethiol and 1-decanethiol were used at total 5 mM solutions, in 100 proof Ethanol solution. Resulting SAM layers were investigated for surface corrugation, morphology and structure variation at different thiol ratios. Observations will be discussed, quantitatively and qualitatively. Eventually mixture ratios were so selected towards optimum conditions for confining Staphylococcus epidermidis. SAM surfaces were investigated using intermittent contact, noncontact, lateral force and contact modes of Atomic Force Microscopy (AFM).

Surface engineering applications

243rd National Meeting (2012)

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