We are working to develop artificial lipid bilayer platforms which allow the measurement of physiologically relevant ion channels quickly, easily, and inexpensively. In our initial work, we demonstrated the automated synthesis of lipid bilayer arrays. Our current work centers on measurement of the human cardiac potassium channel hERG (Kv11.1), rat TRPM8, and other physiologically relevant ion channels. In particular, our bilayer apparatus allows solution perfusion, enabling measurement of ensemble ion channel conductance in the presence of varying modulator concentrations, resulting in EC50 and IC50 values that match the literature. I will report on our progress to validate this platform with a number of physiologically relevant ion channels and develop a parallel and automated platform to perform these measurements with increased throughput. Such a platform may find use to increase productivity of conventional lipid bilayer studies as well as high throughput ion channel screening.

Artificial lipid bilayer platforms for ion channel screening

243rd National Meeting (2012)

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