Combining scanning probe microscopy, force spectroscopy and fluorescence microscopy on the same microscope platform provide high sensitivity and spatial resolution that facilitate functional, correlated imaging and molecular level characterization of biological processes. The expression of cell surface marker CD38 on chronic lymphocytic leukemia (CLL) B-cells is heterogeneous and carries a prognostic significance. We aim to identify and quantitatively determine the B-CLL cell surface markers CD19, CD20 and CD38 by fluorescence microscopy and image processing methods. The diagnosis based on the cell imaging results was supported by the flow cytometry results. These multimodal imaging tools allow to image individual molecules and to probe molecular assemblies by AFM, with the advantages of fluorescence microscopy for the selective and specific visualization of labelled molecules in bacteria and mammalian cancer cells.

Correlated imaging and nanomechanical force mapping

243rd National Meeting (2012)

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