Our presentation will describe the development and preparation of a new class of materials for surface enhanced Raman scattering (SERS) comprised of gold nanoparticles coated onto buoyant silica microbubbles.  These materials allow create a new type of molecular assay designated as a Lab on a Bubble (LoB).  LoB materials serve as a convenient platform for the detection of analytes in solution and offer advantages over traditional colloidal gold and planar SERS substrates.  An example assay is presented using LoB materials and cyanide detection. Cyanide binds to SERS-active, gold-coated LoBs and is detected directly from the corresponding SERS signal.  A second example, a sandwich LoB assay for cholera toxin (CT) will be presented.  The CT assay is composed of antibody modified silica bubbles and modified silica coated nanoparticle reporters.  Our current detection level for CT is ~ 2000 ng where the assay format is a mass determination -- independent of the sample volume.

Lab on a bubble (LoB): Separations and analysis on buoyant SERS bubbles

243rd National Meeting (2012)

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