It was revealed that the genomes of 25 Shewanella strains hold most genes related to the glycolytic pathways; however, they are not capable of utilizing six-carbon carbohydrates (e.g. glucose) because phosphorylating enzymes of glycolysis are not found in their genomes. Not only lacking of key enzymes, Shewanella strains also have only one set of phosphotransferase system to uptake simple sugars. In this study, we introduced two genes, glucokinase (glk) and glucose facilitator (glf) of Zymomonas mobilis, into Shewanella strains to enhance the ability for glucose uptake and utilization. And we considered activating EMP pathway for glycolysis of Shewanella strains with phosphofructokinase (pfk) of Escherichia coli K-12. Glucose consumption and cell growth were determined in the glucose containing medium with/without electron acceptors indicating the recombinant Shewanella could utilize glucose as sole carbon and energy sources. This strategy can be complemented the lacking parts of glucose utilization of Shewanella strains.

Metabolically engineered <i>Shewanella</i> strains using glucose as sole carbon and energy sources

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Metabolically engineered Shewanella strains using glucose as sole carbon and energy sources