Consolidated bioprocessing (CBP) is believed to be a potentially cost-efficient and commercially viable way to produce cellulosic biofuels. But the spontaneous crystallization of cellulose and high degree of hydrogen bonding can form densely packed micro-fibrils that are inaccessible to cellulolytic enzymes. Therefore, efficient and cost-effective methods for the degradation and fermentation of lignocellulosic biomass to hydrogen are required. In this work, for deconstruction of biomass, the ammonia fibre expansion (AFEX) was used as a pre-treatment medium, and the efficient degradation and assimilation process was investigated. This effective process has been named as AFEX-based Consolidated Bio-Processing. In this process, pretreated biomass by AFEX could be hydrolyzed to glucose and xylose and then directly converted to hydrogen by functional consortium that were simultaneously displayed endonuclease, exonuclease, �-glucosidase, and xylanase on the cell surface. Research findings from these studies will be presented.

Development of AFEX-based consolidated bioprocessing for biohydrogen production

243rd National Meeting (2012)

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