Two types of Shiga toxins have been shown to be the major causative agents of Hemolytic Uremic Syndrome caused by the Shiga toxin-producing bacteria E. coli O157:H7, Shiga toxin type 1 (Stx1) and Shiga toxin type 2 (Stx2). E. coli expressing the latter are reported to be of greatest clinical significance.  A crystal structure for Stx1 with bound ligand has facilitated design of STARFISH and PolyBait molecules, which afford protection against the effects of this toxin. However, the crystal structure of Stx2 has only been solved in the absence of bound ligand and neither of the aforementioned ligands affords protection against Stx2. We report a series of studies to correlate the different ligand preferences of Stx2 and an approach for the design and evaluation of heterobifunctional ligands that neutralize Stx2.

Heterobifunctional ligands to bind and neutralize the <i>E. coli</i> shiga like toxin, Stx2

243rd National Meeting (2012)

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Heterobifunctional ligands to bind and neutralize the E. coli shiga like toxin, Stx2