Endocrine disrupting chemicals (EDCs), that are often found in municipal wastewater, compromise development and/or sexual maturation of aquatic organisms by mimicking or antagonizing the actions of natural hormones. A bioassay using a model organism can be a powerful tool as it enables us not only to predict presence of EDCs in water through biological responses but also to assess potential impacts on aquatic organisms due to EDCs exposure.  We investigated gene expression pattern of medaka larvae exposed to six prototypic chemicals (17- Estradiol, Faslodex; ICI 182,780, 11-ketotestosterone, Flutamide, 3,3', 5-Triiodo-L-thyronine, and Amiodarone) using in-house medaka microarray chip containing DNA probes to EDC exposure responsive genes. Water samples obtained from waste water treatment plant in California was utilized to demonstrate EDC classification power of the method.  In our presentation, we will discuss the possibility of screening, classification and identification of uncharacterized EDCs in ambient water based on our microarray analysis.

Classification and identification of endocrine disrupting chemicals through bioassay in quart medaka

243rd National Meeting (2012)

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