Quantitative mass spectrometry-based methods are being developed at the U.S. FDA to detect food allergens and guide regulatory decisions authorized by the Food Allergen Labeling and Consumer Protection Act of 2004. Although allergen detection methods using isotopically labeled peptides are rapidly maturing, the field is still in its infancy. Standard methods are not available to address the large variety of food matricies encountered in regulatory analyses. Use of a sample extraction protocol or selected reaction monitoring (SRM) list optimized for one model food product does not guarantee its effectiveness when applied to another, ostensibly very similar, product. For this reason, each new sample has the unwanted potential to require embarking on a method development research project and the costs of developing these botique methods may be prohibitive. A case study of the development of an SRM method to measure the concentration of milk incurred in baked biscuits prepared using different recipes is described. Intact 15N-labeled a-S1-casein protein and 13C15N-labeled peptide standards were used to measure milk at low ppm levels in allergen ELISA proficiency test samples. The use of intact isotopically labeled protein allergens as recovery and quantitation internal standards is a general strategy for economically managing food composition diversity in regulatory analyses.

Quantitative analysis of food allergens: A case study of the selected reaction monitoring of α-S1-casein in baked goods using an intact <sup>15</sup>N-labeled protein internal standard

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Quantitative analysis of food allergens: A case study of the selected reaction monitoring of α-S1-casein in baked goods using an intact 15N-labeled protein internal standard

Quantitative analysis of food allergens: A case study of the selected reaction monitoring of α-S1-casein in baked goods using an intact ¹⁵N-labeled protein internal standard