Alcohol-induced liver injury progresses from fatty infiltration followed by inflammation leading to an irreversible damage. In this study, two compounds (emodin and chrysophanol) isolated from marine fungus Aspergillus. sp were examined for their protective effects against ethanol-induced toxicity in vitro.  Ethanol-induced HepG2/CYP2E1 cells were treated with the compounds at various concentrations and the results showed that there was a dose-dependent decrease of Gamma-Glutamyl transpeptidase (GGT) activity and increase of glutathione (GSH) in the culture media with an increase in cell viability. Furthermore, the protective effects of the compounds were evaluated by protein expression levels of GGT, GSH and CYP2E1 using Western blot. Among the compounds, emodin addressed to the ethanol induced cytotoxicity more effectively than chrysophanol. It was suggested that emodin isolated from this genus would be a potential candidate to attenuate ethanol induced liver damage with further industrial applications such as functional food and pharmaceutical applications.

Emodin and chrysophanol isolated from marine fungus (Aspergillus. Sp) had protective effects on ethanol-induced toxicity in HepG2/CYP2E1 cells

243rd National Meeting (2012)

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