A device was developed to detect fermenting and non-fermenting bacterial contamination of products with the majority of bacteria detected in <24 hr.  The device consists of a new Neogen Soleris� vial system containing a mixture of carbon dioxide indicators homogeneously dispersed in a semi-permeable matrix separated by a gas permeable membrane from a chamber containing growth media and sample.  The carbon dioxide indicators are located in a region of the device with an optically transparent window that permits spectroscopic detection of the absorbance change that occurs upon detection of carbon dioxide produced from bacterial metabolism.  The device was able to detect in < 24 hr a panel of microorganisms that were inoculated between 10 and 100 CFU/mL into the vial's growth media. In addition, detection speed was linearly correlated to bacterial load in UHT milk samples inoculated from 109 to 102 CFU/mL.

Development of a new device for rapid detection of total aerobic mesophilic bacteria

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.