Stem cells provide valuable resources for both therapeutic treatments and research in fields such as tissue engineering, pathology, and developmental biology.  As these applications expand, improvements in stem cell expansion must be developed.  Fed-batch bioreactors allow for increased volumetric scale-up and batch-to-batch reproducibility; however, the sodium bicarbonate buffers utilized in current medias require carbon dioxide sparging to maintain pH.  Besides complicating system controls, carbon dioxide sparging can reduce oxygen supplies, which in turn can prevent higher cell densities from being obtained.  This study developed a buffer that de-emphasizes sodium bicarbonate's contributions, which eliminates the need for carbon dioxide sparging.  Without any adaptation period, murine mesenchymal stem cells cultured with HEPES-MOPS buffer in atmospheric carbon dioxide grew at rates equal to those achieved in high carbon dioxide environments.  Retention of multipotency and differentiation patterns for stem cells, and application to suspension cultures will be discussed.

Development of buffer supporting mesenchymal stem cell growth in atmospheric carbon dioxide

243rd National Meeting (2012)

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