The regeneration of complete osteochondral constructs with a physiological structure is a significant issue. In this study, a method that used bone marrow stromal cells(BMSCs) with silk/self-assembly peptide scaffold and cultured in a two-chamber well  for one step osteochondral multilayered constructs generation in vitro was investigated. This co-culture system could provide osteogenic and chondrogenic stimulations to BMSCs concurrently on the different layers of scaffold. Results demonstrated that this co-culture approach could successfully provided zones differently chemical stimulations to BMSCs on different layers in a single scaffold, which resulted the formation of the multilayered osteochondral constructs with cartilage like, osteochondral interface and subchondral bone like tissues. Cells at intermediated region appeared hypertrophic chondrocytes morphology . These cells embed in calcified ECM which was also rich in glycosaminoglycans, type collagen I, II and X. In conclusion, this study provided a one-step approach that choosing BMSCs as only cell sources seeded on single scaffold for osteochondral multilayered constructs regeneration.

Generation of osteochondral multilayered constructs by bone marrow stromal cells in vitro

243rd National Meeting (2012)

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