Monoclonal antibodies are typically purified with a platform approach where capture using affinity chromatography with Protein A has become the industry standard. However, for antibody fragments there is not yet a corresponding solution.Here we will discuss purification challenges for antibody fragments and will present several chromatography media alternatives suitable for purification of antibody fragments of different subclasses, size, and structure.Application examples showing the purification of antibody fragments using affinity media will be shown. Screening and optimization of elution conditions, binding capacities, yield and ligand leakage data will be presented, as well as challenges and opportunities of a platform purification solution for antibody fragments.

Affinity platforms for the purification of antibody fragments

243rd National Meeting (2012)

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