Hexapeptide ligands HWRGWV, HYFKFD and HFRRHL identified by our group show high affinity for the Fc fragment of IgG. In this work we demonstrate the applicability of these peptide-based adsorbents to purify antibodies from cell culture supernatants, transgenic plant extract and milk. The purity and yield of the MAbs recovered from CHO supernatants were higher than 94% and 85% respectively. The HWRGWV ligand was also used for purification of MAb from plant extract. To prevent fouling of chromatographic media by pigments and phenolic compounds, the plant extract was pre-treated with a hydrophilically modified charcoal. This treatment removed pigments and phenolic compounds without significantly affecting the MAb concentration. The treated plant extract was then subjected to affinity purification resulting in MAb purity and yield of 96% and 90%. Finally, the peptide adsorbent was used to purify antibody from milk and whey resulting in IgG purity and yield of 95% and 85%.

Affinity purification of antibodies from biological fluids using Fc-binding peptide ligands

243rd National Meeting (2012)

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