The concept of artificial extracellular matrix (ECM) is very important approach in tissue engineering fields due to its significant biological activities. However, it is required that development of facile and efficient technique for multicomponent coating on artificial ECM surface. Recently, we reported artificial ECM mimics based on fusion of mussel adhesive protein (MAP) with the biofunctional ECM peptides. Adhesive properties of MAP enabled efficient immobilization of ECM peptides without any protein and/or surface modifications, which significantly enhanced cellular behaviors on each ECM mimics. Here, we easily constructed multicomponent artificial ECM environment through simple combination of several artificial ECM mimics. Diverse biological activities such as adhesion, proliferation, and differentiation on artificial ECM mimic mixture-coated surfaces were investigated for several bone cell lines. We found that multicomponent artificial ECM mimics showed superior abilities on cells to single component ECM mimics. Thus, multicomponent artificial ECM based on biofunctional peptide-conjugated MAP can be successfully applied in tissue engineering.

Mussel adhesive protein-based multicomponent artificial extracellular matrix mimics for bone tissue engineering

243rd National Meeting (2012)

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