As petroleum-based feeds continue to be depleted, industries that rely on them have an increased pressure to pursue biorenewable means. As part of the Center for Biorenewable Chemicals (CBiRC), we are investigating polyketide biosynthesis pathways in Saccharomyces cerevisiae as a source of renewable carbon precursors. By coupling enzyme engineering, enzyme expression systems, and robust pathway engineering, our goal is to achieve high-level synthesis of cyclic pyrones in S. cerevisiae. Initially, by combining the most promising synthases with strains engineered for improved expression of enzymes and precursors, we have increased titer 15-fold and yield by 30-fold (g/g glucose). Continuing to engineer S. cerevisiae's pathways and enzyme expression systems will lead to an attractive biocatalyst for the biorenewable chemicals industry.

Development of a robust <i>S. cerevisiae</i> strain for the high-level synthesis of pyrones

243rd National Meeting (2012)

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Development of a robust S. cerevisiae strain for the high-level synthesis of pyrones