Because of the advantage of allowing screening of clones in suspension culture at the earlier stage, many companies recently introduced 24 deepwell plate batch culture for stable cell line development, especially for screening and expanding desirable clones. Screening of the clones in suspension culture is more predictive than those in static culture.  However, screening of the clones in batch mode ignores the response of clones to the nutrient feeding applied in fed-batch production,  therefore, may lead to loss of the best potential clones.   In this work, we investigated a feasibility of 24 deepwell culture in a fed-batch mode for early screening of clones in the GS-CHO cell line development. We developed the feeding strategy for the 24 deepwell plate fed-batch cultures and compared the cell growth and antibody production in 24 deepwell plates to those in shake flasks. The results show similar cell growth and antibody production in both 24 deepwell plates and shake flasks.  Our data demonstrates that application of the 24 deepwell fed-batch cultures provides a better high-throughput screening for cell line development in terms of increasing screening throughput and reducing shaker space and medium volume required by shake flasks.

Application of 24 deepwell plate fed-batch cultures for high throughput screening in stable cell line development

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.