Combinatorial design approaches to improve enzymatic or microbial production of a metabolite require high-throughput and selective screening systems. Regulatory proteins controlled by �effector� molecules naturally couple molecular recognition to changes in gene expression, providing a platform for linking in vivo molecular synthesis to a readily detectable phenotype (e.g. GFP expression). We are developing customized molecular reporters by engineering regulatory protein effector recognition. In addition to enabling high throughput screening, customized regulatory proteins are useful tools in metabolic engineering applications.Our success in using AraC variants as endogenous reporters has motivated similar efforts with other regulatory systems. The TetR  repressor is well suited for detecting polycylic natural products. This  poster describes our progress in optimizing and screening TetR  libraries to design novel reporters for various polyketides.

Novel endogenous molecular reporters from engineered TetR regulatory protein

243rd National Meeting (2012)

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