n-Butanol is an important industrial chemical and next-generation biofuel. Bioprocessing of renewable resource to n-butanol is environmentally friendly, but hampered by the low n-butanol titer and consequently high recovery cost. A mutant strain of Clostridium achieved by adaptive evolutionary engineering could produce 21 g/L and 28 g/L n-butanol in free and immobilized cell fermentations, respectively. The mutant and its parent strain were used to reveal the mechanisms of improved n-butanol tolerance and production by comparative proteomic and genomic analyses. The proteomic profiles of both strains were analyzed by 2-D gel electrophoresis. Proteins with dramatic expression difference were identified and classified. Complete genomes of both strains were sequenced using the high-throughput Illumina sequencing technology and analyzed. SNP and Indel analyses revealed 7 variations contributing to the characteristics of the mutant strain. The results can be used in further strain improvement via inverse metabolic engineering.

Mechanisms of improved n-butanol tolerance and production revealed by comparative proteomic and genomic analyses of a mutant strain of Clostridium

243rd National Meeting (2012)

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