Ligncellulosic biomass is abundant and more economical for use in fermentation to produce butanol, one promising biofuel. However, ligncelluloses need to be hydrolyzed into monosaccharides before fermentation. The hydrolysis process usually produces some inhibitory compounds that could severely inhibit bacteria growth and butanol production. Therefore, the inhibitors in the hydrolysate must be reduced or removed by certain detoxification processes before fermentation. To make the detoxification process more efficient, a better understanding of these inhibitors and their inhibition effects is needed. In this study, we investigated the inhibition effects of various inhibitors derived from degradation of carbohydrates and lignin on several solventogenic Clostridia strains. Tolerance and fermentation kinetics of C. beijerinckii and C. acetobutylicum exposed to these inhibitors at various levels were determined, and the information was then used to optimize both hydrolysis and detoxification processes.  The improved hydrolysates significantly increased butanol titer, productivity and yield in the fermentation.

Effects of lignocellulose derived inhibitors on butanol fermentation of<i> C. beijerinckii</i> and <i>C. acetobutylicum</i>

243rd National Meeting (2012)

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Effects of lignocellulose derived inhibitors on butanol fermentation of C. beijerinckii and C. acetobutylicum