Glycopolymer brushes with different spatial distribution of epitopes were successfully prepared on the SPR chips. The interaction between lectin and carbohydrate residues in brushes were evaluated by SPR and AFM force spectroscopy. The apparent affinity constant, sensitivity to change of solution, unbinding force between Con A and carbohydrate residues in brushes show dependence on the composition, not on grafting density. The brushes with different mannose contents exhibited the affinity constant from 1.8 X 104 to 2.2 X 106 M-1, , unbinding force from 51.3 pN to 171 pN, , which is characteristic for monovalent and multivalent interaction between Con A and mannose. The difference in affinity constant and unbinding force is attributed to not only the effective concentration of the ligand in the close proximity of the binding sites of Con A but also the steric interference arising from the inert galactose units.

Effect of spatial distribution of epitopes within glycopolymer brushes on the binding of Con A: From monovalent to multivalent interaction

243rd National Meeting (2012)

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