In this paper we report results of UV-Visible, Raman and Infrared spectroscopy of nanoparticle containing electrophoresis media - namely, aqueous agarose and polyacrylamide gels with and without protein components. Our early results show promise for the detection and possible speciation of unlabeled proteins without their removal from the elcrophoresis media. Detection sensitivity is poorer than conventional plate reading with labeled proteins. But this situation may be further improved using immunological style sandwich type assays to improve the intimacy of contact between nanoparticles and target proteins.

Nanoparticle enhanced spectroscopy proteins in gel electrophoresis media: UV-visible, Raman and infra-red measurements

243rd National Meeting (2012)

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