Proteins are markers for a wide variety of applications, including the detection of allergens in food samples at the point-of-need and bioterrorism agents in-field.  For such applications, a sensitive, yet rapid and inexpensive, detection assay that requires minimal training and power is desired.  Due to its ease of use, rapid processing, and minimal power requirements, the lateral-flow immunoassay (LFA) represents an attractive detection method for these applications.  However, the detection limit of LFA is inferior to lab-based assays, such as the enzyme-linked immunosorbent assay, and needs to be improved.  In a proof-of-concept study, we investigated utilizing aqueous two-phase systems to concentrate a model protein prior to the detection step, and in a predictive manner, improved the detection limit of LFA.

Enhancing the rapid detection of protein markers using aqueous two-phase complex fluid systems

243rd National Meeting (2012)

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