In the present study, epigallocatechin gallate (EGCG) was encapsulated in novel nanoparticles assembled from bioactive peptides, caseinophosphopeptides (CPPs), and chitosan (CS), with the particle size of 150 � 4.3 nm, surface charge of 32.2 � 3.3 mV. Cross-linking between the --NH3+ groups of CS with the -P=O- and -COO- groups of CPPs, as well as the hydrogen bonding were confirmed from the FT-IR spectra. Atomic force microscopy (AFM) images showed that EGCG loaded CS-CPP nanoparticles were spherical in shape. Maintaining the surface charge as high as +32.2mV, cross-linking CS with peptides eliminated the cytotoxicity of CS nanoparticles. In addition, cellular internalization of EGCG-loaded CS-CPP nanoparticles was confirmed from green fluorescence inside the Caco-2 cells. The process of nanoparticle uptake was dose and time dependent in the studied time and concentration range. Furthermore, the intestinal permeability of EGCG was enhanced significantly as delivered by the nanoparticles, which indicated the promising elevation of EGCG bioavailability.

Cellular toxicity, cellular uptake and intestinal absorption enhancement effect of chitosan-caseinophosphopeptides nanoparticles loaded with epigallocatechin gallate

243rd National Meeting (2012)

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