Immuno-isolation of secretory cells in a semi-permeable capsule continues to be a method of interest for cell-based enzyme and hormone replacement therapies. Our group looks to improve the oldest and most widely investigated type of capsule system, the calcium-Alginate-Poly-L-lysine-Alginate (APA) capsule, by replacing the exterior layer of alginate with a Temporarily Reactive Polyelectrolyte (TPR). APA capsules are held together by weak ionic interactions that dissipate overtime in the body exposing the encapsulated cells. A TRP is a polyanion that possess reactive electrophilic groups capable of forming permanent amide covalent crosslinks with the underlying poly-L-lysine, improving the capsules long term stability. Additionally, the TRPs are designed to subsequently hydrolyze increasing the net negative charge of the polyanion by liberating carboxylates, which is speculated to further improve biocompatibility of the capsules

Temporarily reactive polyelectrolytes for cell encapsulation

243rd National Meeting (2012)

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