Cells respond to the chemical and physical properties of their environment through membrane receptors.  One challenge in this area pertains to investigating receptor response to the physical properties of a signaling ligand in situ.  Herein, we describe the development of a photoactivatable platform to dynamically control the immobilization of biomolecules to a supported lipid membrane with high spatial and temporal resolution.  This system employs photocleavable DNA tethers that are easily multiplexed and labeled with ligands of interest to selectively release anchored biomolecules from a lipid membrane surface.  Biotin-streptavidin anchoring chemistry is used to incorporate oligonucleotide capture strands that are functionalized with a 5'-2-nitrophenyl group into the membrane.  These strands are complementary to cyclic RGD peptide conjugated oligonucleotides that engage adhesion receptors in cells, and can be released upon photo-irradiation.  This presentation will describe the synthesis and properties of these model membranes and their potential use in studying dynamic cell adhesions.

Dynamic control of hybrid cell-supported lipid membrane junctions

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.