The carotenoid cleavage dioxygenase (CCD) family represents an attractive enzyme pool for the production of natural bioactives. However, industrial application of CCDs as biocatalysts is hindered by a number of difficulties. Most importantly, hydrophobic carotenoids need to be delivered to the enzymes which require aqueous reaction media. With AtCCD1 from A. thaliana as our model enzyme we investigated the effect of different vesicle based carotenoid delivery systems on substrate specificity and activity. A systematic study was carried out by varying parameters important to micelle and liposome based substrate delivery systems, e.g. surfactant structure, organic co-solvent share and phospholipid composition. Our findings are not only useful for tailoring biocatalytic process conditions but also for a better understanding of the molecular mechanisms involved in substrate recruitment by CCDs. Finally, first results on carotenoid-rich cyanobacterial biomass being a suitable technical substrate for AtCCD1 based formation of natural beta-ionone will be presented.

Toward improved in vitro performance of carotenoid cleavage dioxygenases

243rd National Meeting (2012)

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