Mutli-platform analysis provides increased coverage of the plant metabolome, allowing deeper insight into bio-molecular processes representative of a plant's stress response.  We used both gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) to investigate the metabolic influence of a single gene in rice.  Two rice varieties, one containing SUB1A (flooding tolerant) and one without SUB1A (flooding intolerant) were submerged and the aerial tissue analyzed. NMR quantified 16 metabolites, 9 of which had significant differences between the two varieties during and after submergence, while GC-MS analysis quantified 32 metabolites of which over 20 were significantly different between rice varieties.  Despite the higher number of metabolites detected by GC-MS, 2 metabolites were present only in the NMR data, one of which had not been previously characterized in the rice metabolome by either platform.  The combination of NMR and GC-MS provides greater metabolite coverage and increased confidence in the changes observed.

Multi-platform metabolomics:  Combining NMR and GC-MS to give a deeper understanding of the rice metabolome

243rd National Meeting (2012)

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