Fibrinogen is a primary glycoprotein in blood and involves in coagulation, ability to promote platelet adhesion and aggregation. Responses of blood to implant materials are investigated in vitro. The first reaction of blood to foreign materials is protein absorption (PA). The PA of human fibrinogen (hFg) onto poly (methyl methacrylate), PMMA is ~ 0.05% of its concentration. Biocompatibility of PMMA using water-contact-angle is ~ 78 degrees angle. Atomic force microscopic (AFM) images showed three nodes of each fibrinogen molecule. To form fibril, fibrinogen stacked from middle-to-end through alpha C. The modulus of nodes and connecting points in fibrin are investigated by liquid AFM. The Fourier-transform infrared (FTIR) spectra at 1650 and 1625 cm-1 are alpha helix and beta sheet, respectively. Finally, human endothelial cells are cultured on hFg deposited PMMA surface. Confocal microscopy reveals alpha C involved in cell connections which brings understanding to regenerative vascular vessels.

Fibrinogen and regenerative vascular artery

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.