The use of enzymes in organic solvents offers a great opportunity for the synthesis of complex organic compounds and is therefore in focus of current research. In this work we describe the synthesis of poly-2-methyl-1,3-oxazoline and poly-2-ethyl-1,3-oxazoline enzyme conjugates with hen-egg white lysozyme and a-Chymotrypsin by a new coupling technique. The POXylation was carried out reacting pyromellitic acid dianhydride subsequently with ethylenediamine terminated POx and then with the NH2-groups of the respective enzymes. Upon conjugation lysozyme became fully soluble in DMF (1.4 mg/ml). These are the first examples of fully POXylated proteins, which become organosoluble. The synthesized enzyme conjugates were characterized by SDS-PAGE and dynamic light scattering all indicating the full POXylation of the enzymes. The modified enzymes even partly retained their activity in water. With a-Chymotrypsin we could demonstrate that the molecular weight of the attached polymer significantly influences the activity depending on the size of the substrate.

Organosoluble polymer enzyme conjugates with poly(oxazoline)s

243rd National Meeting (2012)

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