Biofuel production in microorganisms has great potential to fulfill this nation's need for fungible �green� transportation fuels.  Because of the simplicity of bacterial cells, photosynthetic cyanobacteria make good candidates as a platform for biofuel production.   Synechocystis sp. PCC 6803 is an attractive production platform due to rapid doubling times, a sequenced genome and an established molecular biology toolbox.  This molecular biology toolbox was established to probe the biology of photosynthesis and has not been optimized for pathway engineering which is necessary for biofuels production.  This talk will discuss our efforts to develop these tools.  In particular, it will focus on our efforts to develop alternative counter selection markers (to transform wild type Synechocystis) and our efforts to develop and characterize promoters for gene expression under varying conditions.

Towards biofuel production in <i>Synechocystis</i> sp. PCC 6803: Expanding the molecular biology toolbox

243rd National Meeting (2012)

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Towards biofuel production in Synechocystis sp. PCC 6803: Expanding the molecular biology toolbox