Developing efficient and biocompatible vector is of great importance for the clinical application of RNA interference. In this study, we developed a stimuli-responsive nanogel based on a biodegradable polyamide backbone made from natural amino acids. Two different nanogel complexes were made from either free polymer or in situ crosslinking. Upon entering the cytoplasm, siRNA encapsulated in the nanogel could be efficiently released in the reducing environment. NIH 3T3 cells expressing green fluorescence protein was used as a model for in vitro transfection.  The cells were pretreated with chloroquine and the fluorescence images showed that cells treated with the in situ crosslinked TetK/siRNA nanogel has greatly diminished fluorescence both in serum-free and 10% serum media, indicating high transfection efficiency. The excellent biocompatibility and high transfection efficiency suggest the current system a promising candidate for siRNA delivery application.

In situ crosslinked biodegradable and stimuli-responsive nanogel for efficient siRNA delivery

243rd National Meeting (2012)

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