The Secretion and Capture Technology (SECANT�) platform utilizes a non-covalent cell surface attachment to display a protein of interest on the surface of yeast for protein engineering applications. With this method, diversified libraries of proteins including complex scaffolds (full-length IgG, Ig derivatives, multispecific antibodies, binding-scaffolds, etc.) can be constructed and screened for binding and non-binding attributes such as improved expression, stability, solubility, reduced aggregation, etc via high-throughput flow cytometry or magnetic-bead sorting.  We describe the novel aspects of the SECANT� platform in conjunction with our fully-human, IgG1 Library for de novo discovery of therapeutic antibodies.  Additionally, we describe how the platform has been used to construct and screen a tandem scFv antibody library incorporating novel forms of diversity for improved binding to a target.    We also demonstrate how the unique nature of the SECANT� platform facilitates the purification and characterization of mutant clones directly from the selection output.

SECANT<sup>®</sup> yeast display platform for discovery of human antibodies, engineering of complex scaffolds, and novel selections of protein therapeutics

243rd National Meeting (2012)

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SECANT® yeast display platform for discovery of human antibodies, engineering of complex scaffolds, and novel selections of protein therapeutics

SECANT^® yeast display platform for discovery of human antibodies, engineering of complex scaffolds, and novel selections of protein therapeutics