UV detection is the traditional method used in flash chromatography to monitor and fractionate peaks during purification. There are few options available to chromatographers to intelligently purify compounds invisible to UV detection. These invisible compounds may not be detected with UV due to the absence of chromophores, or their absorbance may be �lost� in that of the solvents used in flash chromatography. In other cases, the compounds absorption spectrum may be unknown and the user selected an incorrect wavelength to monitor.In order to overcome the limitations that �invisible� compounds pose, advanced detection techniques, including All-Wavelength Collection, Evaporative Light Scattering Detection, and others are becoming more commonly used. These advanced techniques allow users to easily fractionate compounds without the need for follow-up TLC to determine where the purified compound eluted. Applicability of these advanced techniques for Flash Chromatography are demonstrated on several examples for both Natural Products and Synthetic Chemistry.

Advanced detection techniques for flash chromatography

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.