The GAPDH gene is pivotal in the process of glycolysis, as it is the gene for glyceraldehyde-3-phosphate dehydrogenase.  The GAPDH gene from the plants, Brassica oleracea(broccoli)  and Calendula officinalis (marigold), are isolated, sequenced, and characterized.  The GAPDH enzyme is present not only in plants, but also in animals, fungi, bacteria, and protists, and the gene that codes for it is highly conserved and considered a housekeeping gene.  The nucleic acid is extracted, and then using a PCR module, the GAPDH region is amplified.  After purification, the product is ligated into a plasmid vector, and E. coli bacteria, strain HB101 K-12, is transformed with the vector.  The plasmid is then isolated from the bacterial cells and the DNA is sequenced using the primers  pJETF and pJETR.  A bioinformatics analysis is then conducted of the cloned gene to determine the sequence of the GAPDH genes of Brassica oleracea and Calendula officinalis.

Cloning and sequencing of GAPDH from <i>Brassica oleracea</i> and <i>Calendula officinalis</i>

243rd National Meeting (2012)

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Cloning and sequencing of GAPDH from Brassica oleracea and Calendula officinalis