The dengue virus is a positive-sense RNA virus belonging to the Flavivirus genus, and one of the most common causes of arboviral infections in the subtropical regions of the world.  The dengue RNA-dependent RNA polymerase is proposed to bind specifically to the viral genome through interactions with the 5' untranslated region (UTR) known as stem loop A (SLA).  This strand is complementary to a region in the genome 3' UTR, which has been implicated in the genomic RNA cyclization.  The purpose of this project is to examine the interactions of the dengue 5' and 3' UTRs by using biophysical methods. Both the 5' and 3' UTRs' secondary structures were synthesized by in vitro transcription reactions and characterized by using NMR and fluorescence spectroscopy using a 2-amino purine inserted within SLA.

Biophysical analysis of the interactions between the 5'- and 3'-untranslated regions of the dengue virus genome

243rd National Meeting (2012)

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