Wallemia sebi is a slow growing, xerophilic fungi; for this reason, Wallemia does not grow on standard Inhibitory Mold Agar (IMA) plates used to study and isolate other fungi in the environmental laboratory. The objective of the research was to grow and isolate Wallemia to use as a culture sample. The hypothesis was that the DG-18 agar plates, having a lower water and higher sugar content would support growth of the Wallemi but inhibit the growth of other fungi in the sample. The low water, high sugar content of the DG-18 plates did not inhibit growth of the other fungi; any Wallemia in the samples was overtaken. Dilutions were increased to prevent quick overgrowth. A suspected colony was subbed unto another plate, grown, and identified as being Wallemia. Several weeks of subbing onto new plates was required to isolate the Wallemia sebi from other fungi colonies.

Isolation of Wallemia sebi

243rd National Meeting (2012)

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