Enzymes are biological proteins that catalyze specific reactions in their natural environment.  Changing an enzyme's natural environment in terms of polarity often inactivates enzymes.  Calf intestinal alkaline phosphatase does not follow this trend; as it demonstrates a higher degree of activation by the presence of Trifluoroethanol (a polar organic molecule) in carbonate buffer at pH 10.  However, this activation is not seen in a Tris buffer solution at pH 8.  Our goal has been to elucidate the temperature dependence of alkaline phosphatase at pH 10 in carbonate buffer.  We have examined the enzymes behavior at 45�C, 55�C, and 65�C.  We have also extended our research to pH8 in Tris Buffer.

Temperature dependence of activation of calf intestinal alkaline phosphatase in presence of trifluoroethanol

243rd National Meeting (2012)

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