HDAC3 is a histone deacetylase that plays a critical role in transcriptional regulation, cell growth, cell cycle progression, and apoptosis. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. It's important because DNA is wrapped around histones, and DNA expression is regulated by acetylation and de-acetylation. Histone deacetylases catalyze the removal of acetyl groups from lysine residues in histones resulting in transcriptional repression. HDAC3 does not act alone, but as a component of large multi-protein complexes that are coupled with the signaling of stress related factors, which represses transcription when tethered to a promoter. To assess these interactions, we evaluated the protein interactions between N-CoR and GR with HDAC3 by using co-immunoprecipitation (Co-IP) followed by Western Blotting. The cells were treated with dexamethasone (DEX) then Co-IP's were run by immunoprecipitating N-CoR, and Glucocorticoid Receptor (GR). Precipitates were analyzed for HDAC3 and the effect of Dex treatments by using western blots.

HDAC3 interaction with N-CoR and GR using Co-immunopreciptation after the treatment with dexamethasone

243rd National Meeting (2012)

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