The Arabidopsis thaliana genes Male Meiocyte Death1 (MMD1), MMD1-Like1 (MMD1-L1), and MMD1-Like2 (MMD1-L2) encode for Plant Homeo Domain (PHD) proteins, which are thought to play a role in transcriptional regulation. Structural and functional analyses of these proteins were performed in an attempt to better understand their role in the process.  Purification conditions were determined for MMD1.  Successful isolation required Ni-NTA affinity and size-exclusion chromatography and was confirmed by SDS-PAGE.  Attempts to crystallize the MMD1 protein are ongoing.  In vitro histone-binding assays revealed that MMD1 protein interacts with histones, supporting the idea that MMD1 plays a role in transcriptional regulation. MMD1-L1 and MMD1-L2 purification is underway.

Characterization of <i>Arabidopsis</i> MMD1 protein   and its possible role in transcriptional regulation

243rd National Meeting (2012)

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Characterization of Arabidopsis MMD1 protein and its possible role in transcriptional regulation