Mesoporous silica nanoparticles (MSN) have emerged as an attractive class of drug delivery carriers for therapeutic agents. Herein, we explored the covalent immobilization of proteins into MSN to generate a stimulus-response controlled release. First, MSN were functionalized with thiol groups using (mercaptopropyl)-trimethoxysilane. Activated MSN were characterized by XPS, FTIR, and DLS. The model enzyme, carbonic anhydrase, was coupled to Sulfo-LC-SPDP at a ratio of 1:1. Then, the enzyme was covalently immobilized into MSN via disulfide-disulfide interchange. The enzyme was released from MSN using 10 mM glutathione concentration which represents intra-cellular redox concentrations while it remained bound to the MSN at extra-cellular redox conditions (1 �M). The activity of the released enzyme was >80% demonstrating that the protein was still functional and active after immobilization and release. Viability studies were conducted using HeLa cells to establish biocompatibility of the constructs. After 24 hours, the study showed that there was no cytotoxicity.

Stimulus-responsive controlled release system by covalent immobilization of an enzyme into mesoporous silica nanoparticles

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.