Carotenoids are a group of organic pigments produced by photosynthetic organisms. Streptomyces coelicolor is a soil dwelling bacteria and is used in this study to focus on the kinetic aspect of the biosynthesis of carotenoids. S. coelicolor makes carotenoids to guard itself from stressful oxidative environments such as light. The use of high performance liquid chromatography coupled with UV-Vis detector was employed to separate carotenoid standards and mixture samples of extracted S. coelicolor at different time points under light or dark conditions. After column separation, the dry fractions were studied with NMR spectroscopy to identify the specific carotenoids. The chromatogram and NMR spectra provide further insight on the kinds of carotenoids S. coelicolor produces in response to light and how it metabolizes the same carotenoids when the surrounding environment stabilizes. The kinetic data was analyzed to demonstrate the speed and different stages of production from relative hydrophilic and hydrophobic carotenoids.

Kinetic study of carotenoid biosynthesis in <i>Streptomyces coelicolor</i>

243rd National Meeting (2012)

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Kinetic study of carotenoid biosynthesis in Streptomyces coelicolor