While there have been many studies on the diffusion of membrane proteins and lipids in eukaryotic cells which have given insight into the organization of these membranes, little is known to date of their mobility in bacterial membranes, specifically the Gram negative bacteria, Escherichia coli. The E. coli outer envelope consists of inner and outer lipid membranes that are separated by a periplasmic space containing the cell wall. I will describe our recent single molecule studies of diffusion in the inner and outer membranes of live E. coli cells over time-scales ranging from milliseconds to seconds. Deviations from Brownian motion are modeled to develop insight into the fine structure of the E. coli membranes. Further, single molecule imaging and analysis of the mobility of individual fluorescent proteins in the cytoplasm of E. coli will also be discussed in terms of the structure of the cytoplasm as seen by individual molecules.

Single molecule study of molecular mobility in <i>E. coli</i>

243rd National Meeting (2012)

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Single molecule study of molecular mobility in E. coli