The above-mentioned enzymes accomplish the hydroxylation of their respective substrates at an active site that is fully solvent exposed and entails a ca. 10 � electron transfer across the solvent that separates the two copper centers. The combined reactions, of oxygen/substrate activation and long-range electron transfer, occur in a fully coupled manner, meaning that molecular oxygen uptake and hydroxylated product formation occur in a 1:1 manner under all experimental conditions tested thus far. Collaborative experimental and computational approaches, to address the manner is which such tight control can be exerted over a reaction outcome in a seemingly uncontrolled environment, will be discussed.

Ongoing mechanistic enigma of a two-copper enzyme family:  Peptidylglycine alpha-monooxygenase, dopamine beta-monooxygenase, and tyramine beta-monooxygenase

243rd National Meeting (2012)

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