In the past, synthetic organic chemical insecticides based intervention measures for the control of insect pests and disease, resulted in development of insecticide resistance in some medically important of malaria, filariasis and dengue fever. During the last decade, various studies on natural plant products against mosquito and other insects indicate them as possible alternatives to natural insecticides. Research studies reveal the mosquito larvicidal property of Momordica charantia Linn.   against three mosquito species. Fresh and dried leaves of Momordica charantia Linn.  was used in this experiment for the determination of the volatile and essential oil composition. The headspace (HS) volatile compounds of the fresh leaf was analyzed by solid-phase microextraction (SPME) coupled with capillary gas chromatography/mass spectrometry (GC-MS). Also a Soxhlet extraction technique was carried out with methanol, CH3OH and 0.1g of the freeze dried leaves. In the fresh leaf using SPME, six volatiles compounds were positively identified (E)-2-hexenal, (Z)-3-hexen-1-ol, (E)-2-hexen-1-ol, 4-methyl-1-pentanol, hexyl acetate and 2,4-hexadienal. In the Soxhlet extraction technique, 70% of the total compounds were identified in the 32.4mg of the essential oil obtained. Typical compounds that are found in the oil such as alcohols, hydrocarbons and esters were successfully identified.

Characterization of the organic constituents of <i>Momordica charantia Linn</i>. plant leaves by SPME/GC-MS and Soxhlet extraction technique

243rd National Meeting (2012)

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Characterization of the organic constituents of Momordica charantia Linn. plant leaves by SPME/GC-MS and Soxhlet extraction technique